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Ensure that the instrument is correctly earthed when connected to the power supply. The mains plug is on the left hand side of the instrument towards the rear see picture 1 below. The mains plug is on the rear of the pump station see picture below. Ensure that the the needle wash station is correctly earthed when connected to the power supply. Otherwise local disposal regulations are to be observed. The differences in the specifications are due to the accuracy of the gravimetrical measurement, which to a great extent depends on the quality of the balance, as well as on the stability of the environmental conditions pressure, humidity, temperature.
This means that one deck layout may be used for different methods or one method uses different deck layouts, e. Page 6Wuxfwxuh Ri Wkh 8Vhu 6Riwzduh Technically, this editor is a code generator producing a low-level code which is then interpreted by the run control. This low level code is called HSL Hamilton Standard Language , because it provides a common programming approach for all Hamilton instruments. However, specific access rights may be restricted to trained users within the operating system, or by applying write- protection to specific files.
The next tab refers to barcode handling: The different barcodes which are allowed on the instrument can be selected here. Select the type of communication and the com port for communication via RS interface. Select an instrument e. If there is only one instrument installed, the template base definition of a deck layout of that instrument is shown directly.
Page 52 Basically, the deck is freely configurable. The term labware refers here to carriers available from Hamilton as well as microplates from various manufacturers and tip racks. The general procedure is first to add a carrier for a special kind of plate to the deck and then add the plate to the carrier. A carrier features a varying number of locations for the placement of labware, such as tip racks, microplates, etc. To add a carrier to the deck layout, simply double-click on the appropriate track position.
Page 54 diameter and length. The tube carriers are an example of racks which fit directly into the track grid of the Microlab STAR. Page 54 of A dialog window opens up: Select the type of labware from the library and enter the x,y,z coordinates by hand. The User Software enables you to record the x-y-z coordinates of that point and to associate them with the name you have given the location.
The Advanced section allows you to move directly to a position by clicking on Move Probe. Clicking on Key Control brings you back to the previous dialog. A sequence defines the order of containers in a rack. For example, it links the single wells of a microtiter plate together so as to treat them as one plate.
Once the labware positions in the sequence are in the desired order, save the sequence. Saving fixes the order of each row in the grid as the order of the labware positions in the sequence. Shift-left-click on labware position adds a block of positions to the sequence. That means it adds all positions in the rectangle whose first corner has the coordinates of the most recently added position and whose opposite corner has the coordinates of the newly selected labware position.
The sort is applied to the currently selected labware positions, or to the entire sequence if no positions are selected. Given the following sequence across 2 racks, apply the Sort Racks filter, defining a direction across the racks. They can be as complex or as simple as the user desires. Access to a method editor is necessary to define or edit methods. No instrument-specific steps e. Select the layout from the file list and click Open.
Click OK to link method and deck layout. Note that multiple deck layouts may be linked to one method to enable programming of different instruments within one method. Only in the case of two or more instruments linked together is a selection necessary. Therefore, the pipetting task should be reflected within the sequences defined for this step.
In this case, STAR will have to pick up tips prior to the pipette step and eject them after pipetting, using single steps. This is either the aspiration, or the dispense sequence. As long as both sequences are of the same length, this selection does not influence the pipetting if no pipetting error occurs. Another example for this case: All Tubes to Plate s. Reloadable plate sequence to load all tubes to as many plates as needed. Also used for aliquoting. Furthermore, sequence manipulations can be made separately for aspiration and dispense sequences.
If the sequences which this SMART Step is going to work with have been used within this method in preceding steps, the sequence counters can be reset using the two check boxes for initial sequence manipulation. These commands work with autoload and manual load instruments.
Dragging the ORDG command to the method tree opens up a dialog box: The procedure for loading the instrument follows logically from our concept of sequences. It is very similar to the loading step. Here, all carriers involved in the specified sequences are unloaded from the instrument deck.
The idea is that needles can be used like disposable tips without bothering about the organization of the three wash modules of a wash station. For both commands channel patterns can be set manually. You will find that, for each deck layout or instrument you selected, there is now an additional bar containing the instrument-specific steps. The Graphical Method Editor recognizes three types of variables: integers numbers , floats floating point numbers , and strings text variables.
Note that if your method uses a library function, the library in question is indicated in the list of referenced libraries and can't be removed. In this example, the following functions are linked and now made available within the method: Pressure based aspiration monitoring off Pressure based aspiration monitoring on Capacitance based clot detection off This means all 16 channels should aspirate and dispense simultaneously, to allow maximum parallelization and highest pipetting speed.
In this case, the reduced random access space of a channel Microlab STAR does not cause any problems. Instrument steps are shown with a gray background. Here you will find shortcuts to access the configuration and liquid editor. A dialog appears: Select the first method of interest and click on the buttons to add the method to the list on the right-hand side.
A liquid class is a set of parameters specifying the aspiration and dispense behavior appropriate for a given liquid e. At the top, the attributes of the liquid class are shown: Liquid Name, tip type, dispense mode. Opening the Liquid Editor loads liquids defined in the standard liquid configuration file If other liquids are needed from another liquid configuration file, they can be imported.
Click Import liquid s Page of To add a filter plate on the conditioning site of the vacuum box, double-click on position 2 of the vacuum box and select the right filter plate. A list of deck layouts will appear. The Layout window reappears with the selected layout file showing. These are in alphabetical order and are described below. Empty waste command: Empty waste This command turns on the waste pump inside the vacuum box controller so that the waste tray in the conditioning chamber can be evacuated.
Select the chamber over which the carriage is to be moved. The conditioning chamber is in front and the elution chamber in rear. The ramp duration is the time needed to reach the desired vacuum. Drag the parallel process icon. A dialog appears to start parallel process No 1. Enter a name for the parallel process and click OK. The method editor now shows the bifurcation of the normal and the first parallel process. Page The bifurcation within the method editor ends after this step. To process a plate, for example, with the pipetting channels of the microlab STAR, add pipetting steps to the normal process left side of the bifurcation.
Click OK. Double-click on one of the tracks. A dialog box appears. Click on the LLD button: Set the settings according to this example. On dispense, switch off the LLD and dispense to a fixed height 2 mm from bottom : Accept the defaults for the settings under Advanced and for the error settings. Your first method is ready to go. The only difference is that the method is now written using single steps, to illustrate the difference.
You can easily write the method by dragging icons from the toolbox on the left and dropping them in the method window on the right. A window pops up: Click OK to initialize the instrument in the first step. The waste sequence is selected to eject tips from the channel during initialization. For an instrument with autoload option, this command loads the carriers automatically onto the instrument deck during runtime. For a manual load instrument, this command requests the user to load the carriers for runtime.
Select "Automatic" as sequence counting. You may now enter the settings for mixing. A dialog window appears. The lower part of the dialog is exactly the same as for the aspiration step liquid following and mixing settings. In the upper part, accept the default for using the same liquid class as in the aspiration step. Click OK to accept the values. Now your method is complete. Page To create this deck layout, start the Deck Layout Editor by double-clicking on the appropriate icon.
The Sequence Editor opens up. Rubber-band all four tube carriers with the left mouse button. You now see the 96 selected wells of the four carriers. The grid window on the left shows the generated sequence of 96 positions holding all tubes.
Check the input on the summary that appears and click OK. Click OK to add the step. Now your method is ready to use. Click OK to quit the summary. Your method is now ready to go. The sequence should span some or all of the wells in the source microplate s. This threshold — although numeric — is entered as a string. This is because, in the next step, a valid SQL database selection statement is created. Chat with us or contact one of our team member for assistance.